Rapid development of microsatellite markers for Callosobruchus chinensis using Illumina paired-end sequencing.

ABSTRACT:

Background

The adzuki bean weevil, Callosobruchus chinensis L., is one of the most destructive pests of stored legume seeds such as mungbean, cowpea, and adzuki bean, which usually cause considerable loss in the quantity and quality of stored seeds during transportation and storage. However, a lack of genetic information of this pest results in a series of genetic questions remain largely unknown, including population genetic structure, kinship, biotype abundance, and so on. Co-dominant microsatellite markers offer a great resolving power to determine these events. Here, we report rapid microsatellite isolation from C. chinensis via high-throughput sequencing.

Principal findings

In this study, 94,560,852 quality-filtered and trimmed reads were obtained for the assembly of ge

SUBMITTER: Duan CX 

PROVIDER: S-EPMC4023940 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature