Development of a Cucumis sativus TILLinG platform for forward and reverse genetics.

ABSTRACT:

Background

Cucumber (Cucumis sativus) belongs to the Cucurbitaceae family that includes more than 800 species. The cucumber genome has been recently sequenced and annotated. Transcriptomics and genome sequencing of many plant genomes are providing information on candidate genes potentially related to agronomically important traits. To accelerate functional characterization of these genes in cucumber we have generated an EMS mutant population that can be used as a TILLinG platform for reverse genetics.

Principal findings

A population of 3,331 M2 mutant seed families was generated using two EMS concentrations (0.5% and 0.75%). Genomic DNA was extracted from M2 families and eight-fold pooled for mutation detection by ENDO1 nuclease. To assess the quality of the mutant collectio

SUBMITTER: Boualem A 

PROVIDER: S-EPMC4024006 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature