Astroglial-axonal interactions during early stages of myelination in mixed cultures using in vitro and ex vivo imaging techniques.

ABSTRACT:

Background

[corrected] Myelination is a very complex process that requires the cross talk between various neural cell types. Previously, using cytosolic or membrane associated GFP tagged neurospheres, we followed the interaction of oligodendrocytes with axons using time-lapse imaging in vitro and ex vivo and demonstrated dynamic changes in cell morphology. In this study we focus on GFP tagged astrocytes differentiated from neurospheres and their interactions with axons.

Results

We show the close interaction of astrocyte processes with axons and with oligodendrocytes in mixed mouse spinal cord cultures with formation of membrane blebs as previously seen for oligodendrocytes in the same cultures. When GFP-tagged neurospheres were transplanted into the spinal cord of the dysmye

SUBMITTER: Ioannidou K 

PROVIDER: S-EPMC4024314 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature