Detection of cancer DNA in plasma of patients with early-stage breast cancer.

ABSTRACT:

Purpose

Detecting circulating plasma tumor DNA (ptDNA) in patients with early-stage cancer has the potential to change how oncologists recommend systemic therapies for solid tumors after surgery. Droplet digital polymerase chain reaction (ddPCR) is a novel sensitive and specific platform for mutation detection.

Experimental design

In this prospective study, primary breast tumors and matched pre- and postsurgery blood samples were collected from patients with early-stage breast cancer (n = 29). Tumors (n = 30) were analyzed by Sanger sequencing for common PIK3CA mutations, and DNA from these tumors and matched plasma were then analyzed for PIK3CA mutations using ddPCR.

Results

Sequencing of tumors identified seven PIK3CA exon 20 mutations (H1047R) and three exon 9 muta

SUBMITTER: Beaver JA 

PROVIDER: S-EPMC4024333 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature