Hybrid photopatterned enzymatic reaction (HyPER) for in situ cell manipulation.

ABSTRACT: The ability to design artificial extracellular matrices as cell-instructive scaffolds has opened the door to technologies capable of studying the fate of cells in vitro and to guiding tissue repair in vivo. One main component of the design of artificial extracellular matrices is the incorporation of biochemical cues to guide cell phenotype and multicellular organization. The extracellular matrix (ECM) is composed of a heterogeneous mixture of proteins that present a variety of spatially discrete signals to residing cell populations. In contrast, most engineered ECMs do not mimic this heterogeneity. In recent years, photo-deprotection has been used to spatially immobilize signals. However, this approach has been limited mostly to small peptides. Here we combine photo-deprotection with enzym

SUBMITTER: Griffin DR 

PROVIDER: S-EPMC4024472 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature