PDZ affinity chromatography: a general method for affinity purification of proteins based on PDZ domains and their ligands.

ABSTRACT: PDZ (PSD-95, DiscsLarge, ZO1) domains function in nature as protein binding domains within scaffold and membrane-associated proteins. They comprise ∼90 residues and make specific, high affinity interactions with complementary C-terminal peptide sequences, with other PDZ domains, and with phospholipids. We hypothesized that the specific, strong interactions of PDZ domains with their ligands would make them well suited for use in affinity chromatography. Here we describe a novel affinity chromatography method applicable for the purification of proteins that contain PDZ domain-binding ligands, either naturally or introduced by genetic engineering. We created a series of affinity resins comprised of PDZ domains from the scaffold protein PSD-95, or from neuronal nitric oxide synthase (nNOS), co

SUBMITTER: Walkup WG 

PROVIDER: S-EPMC4024478 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature