Protein kinase A regulates gene-specific translational adaptation in differentiating yeast.

ABSTRACT: Cellular differentiation is driven by coordinately regulated changes in gene expression. Recent discoveries suggest that translation contributes as much as transcription to regulating protein abundance, but the role of translational regulation in cellular differentiation is largely unexplored. Here we investigate translational reprogramming in yeast during cellular adaptation to the absence of glucose, a stimulus that induces invasive filamentous differentiation. Using ribosome footprint profiling and RNA sequencing to assay gene-specific translation activity genome-wide, we show that prolonged glucose withdrawal is accompanied by gene-specific changes in translational efficiency that significantly affect expression of the majority of genes. Notably, transcripts from a small minority (<5%)

SUBMITTER: Vaidyanathan PP 

PROVIDER: S-EPMC4024644 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature