Down-regulated γ-catenin expression is associated with tumor aggressiveness in esophageal cancer.

ABSTRACT:

Aim

To evaluate the significance of γ-catenin in clinical pathology, cellular function and signaling mechanism in esophageal squamous cell carcinoma (ESCC).

Methods

The mRNA expression of γ-catenin was detected by real-time quantitative reverse transcription-polymerase chain reaction in 95 tissue specimens and evaluated for association with the clinicopathologic characteristics and survival time of patients with ESCC. siRNAs against human γ-catenin were used to inhibit γ-catenin expression. Hanging drop aggregation assay and dispase-based dissociation assay were performed to detect the effect of γ-catenin on ESCC cell-cell adhesion. Transwell assay was performed to determine cell migration. Luciferase-based transcriptional reporter assay (TOPflash) was used to measure β-cate

SUBMITTER: Fang WK 

PROVIDER: S-EPMC4024793 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature