Characterization of age-associated alterations of islet function and structure in diabetic mutant cryptochrome 1 transgenic mice.

ABSTRACT:

Aims/introduction

In earlier reports, we described that transgenic (Tg) mice ubiquitously expressing cryptochrome1 (CRY1) with a mutation in cysteine414 (CRY1-AP Tg mice) show an early-onset insulin-secretory defect of diabetes mellitus resembling human maturity-onset diabetes of the young (MODY). To clarify the yet undiscovered molecular pathogenesis of diabetes mellitus in which the mutant of CRY1 is involved, we examined age-dependent characteristics of islets of CRY1-AP Tg mice.

Materials and methods

Immunohistochemical analyses of islets were carried out for 2-, 4- and 19-week-old mice. Insulin contents in the pancreas and glucose-stimulated insulin secretion of isolated islets of mice were measured at 4 weeks. Real-time polymerase chain reaction analyses using pancreas

SUBMITTER: Okano S 

PROVIDER: S-EPMC4025103 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature