Age-associated changes of islet endocrine cells and the effects of body mass index in Japanese.

ABSTRACT:

Aims/introduction

Impaired growth and premature death of β-cells are implicated in the progression of islet pathology in type 2 diabetes. It remains unclear, however, how aging affects islet cells, or whether the islet change in diabetes is an augmented process of aging. We studied age-related changes of the islet structure in Japanese non-diabetic subjects and explored the underlying mechanism of the changes.

Materials and methods

A total of 115 non-diabetic autopsy cases were subjected to morphometric analysis for volume densities of islets, β- and non-β-cells, as well as their masses. Proliferation activity identified by Ki67, and expressions of pancreatic and duodenal homeobox (PDX)-1, cell cycle inhibitor P16, and oxidative stress marker γH2AX were also examined.

Res

SUBMITTER: Mizukami H 

PROVIDER: S-EPMC4025233 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature