A complex regulatory network coordinating cell cycles during C. elegans development is revealed by a genome-wide RNAi screen.

ABSTRACT: The development and homeostasis of multicellular animals requires precise coordination of cell division and differentiation. We performed a genome-wide RNA interference screen in Caenorhabditis elegans to reveal the components of a regulatory network that promotes developmentally programmed cell-cycle quiescence. The 107 identified genes are predicted to constitute regulatory networks that are conserved among higher animals because almost half of the genes are represented by clear human orthologs. Using a series of mutant backgrounds to assess their genetic activities, the RNA interference clones displaying similar properties were clustered to establish potential regulatory relationships within the network. This approach uncovered four distinct genetic pathways controlling cell-cycle entry

SUBMITTER: Roy SH 

PROVIDER: S-EPMC4025478 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature