Macrophages offer a paradigm switch for CNS delivery of therapeutic proteins.

ABSTRACT:

Aims

Active targeted transport of the nanoformulated redox enzyme, catalase, in macrophages attenuates oxidative stress and as such increases survival of dopaminergic neurons in animal models of Parkinson's disease. Optimization of the drug formulation is crucial for the successful delivery in living cells. We demonstrated earlier that packaging of catalase into a polyion complex micelle ('nanozyme') with a synthetic polyelectrolyte block copolymer protected the enzyme against degradation in macrophages and improved therapeutic outcomes. We now report the manufacture of nanozymes with superior structure and therapeutic indices.

Methods

Synthesis, characterization and therapeutic efficacy of optimal cell-based nanoformulations are evaluated.

Results

A formulation desig

SUBMITTER: Klyachko NL 

PROVIDER: S-EPMC4025996 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature