RNA sequencing analysis of the gametophyte transcriptome from the liverwort, Marchantia polymorpha.

ABSTRACT: The liverwort Marchantia polymorpha is a member of the most basal lineage of land plants (embryophytes) and likely retains many ancestral morphological, physiological and molecular characteristics. Despite its phylogenetic importance and the availability of previous EST studies, M. polymorpha's lack of economic importance limits accessible genomic resources for this species. We employed Illumina RNA-Seq technology to sequence the gametophyte transcriptome of M. polymorpha. cDNA libraries from 6 different male and female developmental tissues were sequenced to delineate a global view of the M. polymorpha transcriptome. Approximately 80 million short reads were obtained and assembled into a non-redundant set of 46,533 transcripts (> =  200 bp) from 46,070 loci. The average length and the N50

SUBMITTER: Sharma N 

PROVIDER: S-EPMC4026138 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature