Basic mechanisms of RNA polymerase II activity and alteration of gene expression in Saccharomyces cerevisiae.

ABSTRACT: Transcription by RNA polymerase II (Pol II), and all RNA polymerases for that matter, may be understood as comprising two cycles. The first cycle relates to the basic mechanism of the transcription process wherein Pol II must select the appropriate nucleoside triphosphate (NTP) substrate complementary to the DNA template, catalyze phosphodiester bond formation, and translocate to the next position on the DNA template. Performing this cycle in an iterative fashion allows the synthesis of RNA chains that can be over one million nucleotides in length in some larger eukaryotes. Overlaid upon this enzymatic cycle, transcription may be divided into another cycle of three phases: initiation, elongation, and termination. Each of these phases has a large number of associated transcription factors t

SUBMITTER: Kaplan CD 

PROVIDER: S-EPMC4026157 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature