A synthetic luciferin improves bioluminescence imaging in live mice.

ABSTRACT: Firefly luciferase is the most widely used optical reporter for noninvasive bioluminescence imaging (BLI) in rodents. BLI relies on the ability of the injected luciferase substrate D-luciferin to access luciferase-expressing cells and tissues within the animal. Here we show that injection of mice with a synthetic luciferin, CycLuc1, improves BLI with existing luciferase reporters and enables imaging in the brain that could not be achieved with D-luciferin.

SUBMITTER: Evans MS 

PROVIDER: S-EPMC4026177 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature