Bile acid flux is necessary for normal liver regeneration.

ABSTRACT:

Background & aims

Many signals governing liver regeneration (LR) following 2/3 partial hepatectomy (PH) are recognized, but the primary signal(s) remains unknown. The aim of the study was to confirm that the remnant liver after PH lacks capacity to secrete the BA pool returning via the enterohepatic ciruculation (EHC), which may in turn stimulate LR.

Methods

After standard PH, BA flux was documented and BA signaling (Fgf15) and synthesis (Cyp7a) determined by qPCR. Rat biliary fistula (BF) and Asbt knockout mouse models interrupted the EHC prior to PH, and standard assays for LR employed along with complete RNA sequencing. CCl4 intoxication after BF tested the hypothesis in an alternate injury model.

Results

BA rise in systemic blood immediately following PH, confirmi

SUBMITTER: Naugler WE 

PROVIDER: S-EPMC4026228 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature