Proteomic identification of novel differentiation plasma protein markers in hypobaric hypoxia-induced rat model.

ABSTRACT:

Background

Hypobaric hypoxia causes complex changes in the expression of genes, including stress related genes and corresponding proteins that are necessary to maintain homeostasis. Whereas most prior studies focused on single proteins, newer methods allowing the simultaneous study of many proteins could lead to a better understanding of complex and dynamic changes that occur during the hypobaric hypoxia.

Methods

In this study we investigated the temporal plasma protein alterations of rat induced by hypobaric hypoxia at a simulated altitude of 7620 m (25,000 ft, 282 mm Hg) in a hypobaric chamber. Total plasma proteins collected at different time points (0, 6, 12 and 24 h), separated by two-dimensional electrophoresis (2-DE) and identified using matrix assisted laser desorpti

SUBMITTER: Ahmad Y 

PROVIDER: S-EPMC4026414 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature