Sample-ready multiplex qPCR assay for detection of malaria.

ABSTRACT:

Background

Microscopy and antigen detecting rapid diagnostic tests are the diagnostic tests of choice in management of clinical malaria. However, due to their limitations, the need to utilize more sensitive methods such as real-time PCR (qPCR) is evident as more studies are now utilizing molecular methods in detection of malaria. Some of the challenges that continue to limit the widespread utilization of qPCR include lack of assay standardization, assay variability, risk of contamination, and the need for cold-chain. Lyophilization of molecular assays can overcome some of these limitations and potentially enable widespread qPCR utilization.

Methods

A recently published multiplex malaria qPCR assay was lyophilized by freezing drying into Sample-Ready™ format (MMSR). MMSR assa

SUBMITTER: Kamau E 

PROVIDER: S-EPMC4026594 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature