Local viscoelastic properties of live cells investigated using dynamic and quasi-static atomic force microscopy methods.

ABSTRACT: The measurement of viscoelasticity of cells in physiological environments with high spatio-temporal resolution is a key goal in cell mechanobiology. Traditionally only the elastic properties have been measured from quasi-static force-distance curves using the atomic force microscope (AFM). Recently, dynamic AFM-based methods have been proposed to map the local in vitro viscoelastic properties of living cells with nanoscale resolution. However, the differences in viscoelastic properties estimated from such dynamic and traditional quasi-static techniques are poorly understood. In this work we quantitatively reconstruct the local force and dissipation gradients (viscoelasticity) on live fibroblast cells in buffer solutions using Lorentz force excited cantilevers and present a careful comparis

SUBMITTER: Cartagena A 

PROVIDER: S-EPMC4026794 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature