A dynamic interplay of nucleosome and Msn2 binding regulates kinetics of gene activation and repression following stress.

ABSTRACT: The transcription factor Msn2 mediates a significant proportion of the environmental stress response, in which a common cohort of genes changes expression in a stereotypic fashion upon exposure to any of a wide variety of stresses. We have applied genome-wide chromatin immunoprecipitation and nucleosome profiling to determine where Msn2 binds under stressful conditions and how that binding affects, and is affected by, nucleosome positioning. We concurrently determined the effect of Msn2 activity on gene expression following stress and demonstrated that Msn2 stimulates both activation and repression. We found that some genes responded to both intermittent and continuous Msn2 nuclear occupancy while others responded only to continuous occupancy. Finally, these studies document a dynamic inte

SUBMITTER: Elfving N 

PROVIDER: S-EPMC4027177 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature