Crosstalk between transposase subunits during cleavage of the mariner transposon.

ABSTRACT: Mariner transposition is a complex reaction that involves three recombination sites and six strand breaking and joining reactions. This requires precise spatial and temporal coordination between the different components to ensure a productive outcome and minimize genomic instability. We have investigated how the cleavage events are orchestrated within the mariner transpososome. We find that cleavage of the non-transferred strand is completed at both transposon ends before the transferred strand is cleaved at either end. By introducing transposon-end mutations that interfere with cleavage, but leave transpososome assembly unaffected, we demonstrate that a structural transition preceding transferred strand cleavage is coordinated between the two halves of the transpososome. Since mariner lac

SUBMITTER: Claeys Bouuaert C 

PROVIDER: S-EPMC4027188 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature