Parameters Affecting Image-guided, Hydrodynamic Gene Delivery to Swine Liver.

ABSTRACT: Development of a safe and effective method for gene delivery to hepatocytes is a critical step toward gene therapy for liver diseases. Here, we assessed the parameters for gene delivery to the livers of large animals (pigs, 40-65 kg) using an image-guided hydrodynamics-based procedure that involves image-guided catheter insertion into the lobular hepatic vein and hydrodynamic injection of reporter plasmids using a computer-controlled injector. We demonstrated that injection parameters (relative position of the catheter in the hepatic vasculature, intravascular pressure upon injection, and injection volume) are directly related to the safety and efficiency of the procedure. By optimizing these parameters, we explored for the first time, the advantage of the procedure for sequential injectio

SUBMITTER: Kamimura K 

PROVIDER: S-EPMC4027427 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature