Metal impurities cause false positives in high-throughput screening campaigns.

ABSTRACT: Organic impurities in compound libraries are known to often cause false-positive signals in screening campaigns for new leads, but organic impurities do not fully account for all false-positive results. We discovered inorganic impurities in our screening library that can also cause positive signals for a variety of targets and/or readout systems, including biochemical and biosensor assays. We investigated in depth the example of zinc for a specific project and in retrospect in various HTS screens at Roche and propose a straightforward counter screen using the chelator TPEN to rule out inhibition caused by zinc.

SUBMITTER: Hermann JC 

PROVIDER: S-EPMC4027514 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature