A morpholino-based screen to identify novel genes involved in craniofacial morphogenesis.

ABSTRACT:

Background

The regulatory mechanisms underpinning facial development are conserved between diverse species. Therefore, results from model systems provide insight into the genetic causes of human craniofacial defects. Previously, we generated a comprehensive dataset examining gene expression during development and fusion of the mouse facial prominences. Here, we used this resource to identify genes that have dynamic expression patterns in the facial prominences, but for which only limited information exists concerning developmental function.

Results

This set of ∼80 genes was used for a high-throughput functional analysis in the zebrafish system using Morpholino gene knockdown technology. This screen revealed three classes of cranial cartilage phenotypes depending upon whether

SUBMITTER: Melvin VS 

PROVIDER: S-EPMC4027977 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature