Improving transcriptome construction in non-model organisms: integrating manual and automated gene definition in Emiliania huxleyi.

ABSTRACT:

Background

The advent of Next Generation Sequencing technologies and corresponding bioinformatics tools allows the definition of transcriptomes in non-model organisms. Non-model organisms are of great ecological and biotechnological significance, and consequently the understanding of their unique metabolic pathways is essential. Several methods that integrate de novo assembly with genome-based assembly have been proposed. Yet, there are many open challenges in defining genes, particularly where genomes are not available or incomplete. Despite the large numbers of transcriptome assemblies that have been performed, quality control of the transcript building process, particularly on the protein level, is rarely performed if ever. To test and improve the quality of the automated transc

SUBMITTER: Feldmesser E 

PROVIDER: S-EPMC4028052 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature