A metabolomic approach to animal vitreous humor topographical composition: a pilot study.

ABSTRACT: The purpose of this study was to evaluate the feasibility of a (1)H-NMR-based metabolomic approach to explore the metabolomic signature of different topographical areas of vitreous humor (VH) in an animal model. Five ocular globes were enucleated from five goats and immediately frozen at -80 °C. Once frozen, three of them were sectioned, and four samples corresponding to four different VH areas were collected: the cortical, core, and basal, which was further divided into a superior and an inferior fraction. An additional two samples were collected that were representative of the whole vitreous body. (1)H-NMR spectra were acquired for twenty-three goat vitreous samples with the aim of characterizing the metabolomic signature of this biofluid and identifying whether any site-specific pattern

SUBMITTER: Locci E 

PROVIDER: S-EPMC4028277 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature