Single cell correlation fractal dimension of chromatin: a framework to interpret 3D single molecule super-resolution.

ABSTRACT: Chromatin is a major nuclear component, and it is an active matter of debate to understand its different levels of spatial organization, as well as its implication in gene regulation. Measurements of nuclear chromatin compaction were recently used to understand how DNA is folded inside the nucleus and to detect cellular dysfunctions such as cancer. Super-resolution imaging opens new possibilities to measure chromatin organization in situ. Here, we performed a direct measure of chromatin compaction at the single cell level. We used histone H2B, one of the 4 core histone proteins forming the nucleosome, as a chromatin density marker. Using photoactivation localization microscopy (PALM) and adaptive optics, we measured the three-dimensional distribution of H2B with nanometric resolution. We c

SUBMITTER: Recamier V 

PROVIDER: S-EPMC4028358 | biostudies-literature | 2014 Jan-Feb

REPOSITORIES: biostudies-literature