Soybean peroxidase-mediated degradation of an azo dye- a detailed mechanistic study.

ABSTRACT:

Background

Peroxidases are emerging as an important class of enzymes that can be used for the efficient degradation of organic pollutants. However, detailed studies identifying the various intermediates produced and the mechanisms involved in the enzyme-mediated pollutant degradation are not widely published.

Results

In the present study, the enzymatic degradation of an azo dye (Crystal Ponceau 6R, CP6R) was studied using commercially available soybean peroxidase (SBP) enzyme. Several operational parameters affecting the enzymatic degradation of dye were evaluated and optimized, such as initial dye concentration, H2O2 dosage, mediator amount and pH of the solution. Under optimized conditions, 40 ppm dye solution could be completely degraded in under one minute by SBP in the

SUBMITTER: Ali L 

PROVIDER: S-EPMC4028748 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature