Functional genomics reveals that Clostridium difficile Spo0A coordinates sporulation, virulence and metabolism.

ABSTRACT:

Background

Clostridium difficile is an anaerobic, Gram-positive bacterium that can reside as a commensal within the intestinal microbiota of healthy individuals or cause life-threatening antibiotic-associated diarrhea in immunocompromised hosts. C. difficile can also form highly resistant spores that are excreted facilitating host-to-host transmission. The C. difficile spo0A gene encodes a highly conserved transcriptional regulator of sporulation that is required for relapsing disease and transmission in mice.

Results

Here we describe a genome-wide approach using a combined transcriptomic and proteomic analysis to identify Spo0A regulated genes. Our results validate Spo0A as a positive regulator of putative and novel sporulation genes as well as components of the mature spor

SUBMITTER: Pettit LJ 

PROVIDER: S-EPMC4028888 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature