Characterisation of Caenorhabditis elegans sperm transcriptome and proteome.

ABSTRACT:

Background

Although sperm is transcriptionally and translationally quiescent, complex populations of RNAs, including mRNAs and non-coding RNAs, exist in sperm. Previous microarray analysis of germ cell mutants identified hundreds of sperm genes in Caenorhabditis elegans. To take a more comprehensive view on C. elegans sperm genes, here, we isolate highly pure sperm cells and employ high-throughput technologies to obtain sperm transcriptome and proteome.

Results

First, sperm transcriptome consists of considerable amounts of non-coding RNAs, many of which have not been annotated and may play functional roles during spermatogenesis. Second, apart from kinases/phosphatases as previously reported, ion binding proteins are also enriched in sperm, underlying the crucial roles of in

SUBMITTER: Ma X 

PROVIDER: S-EPMC4028957 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature