DAYSLEEPER: a nuclear and vesicular-localized protein that is expressed in proliferating tissues.

ABSTRACT:

Background

DAYSLEEPER is a domesticated transposase that is essential for development in Arabidopsis thaliana [Nature, 436:282-284, 2005]. It is derived from a hAT-superfamily transposon and contains many of the features found in the coding sequence of these elements [Nature, 436:282-284, 2005, Genetics, 158:949-957, 2001]. This work sheds light on the expression of this gene and localization of its product in protoplasts and in planta. Using deletion constructs, important domains in the protein were identified.

Results

DAYSLEEPER is predominantly expressed in meristems, developing flowers and siliques. The protein is mainly localized in the nucleus, but can also be seen in discrete foci in the cytoplasm. Using several vesicular markers, we found that these foci belong to ve

SUBMITTER: Knip M 

PROVIDER: S-EPMC4029315 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature