Fusion of Hsp70 to Mage-a1 enhances the potency of vaccine-specific immune responses.

ABSTRACT:

Background

Heat shock proteins (HSPs) are capable of promoting antigen presentation of chaperoned peptides through interactions with receptors on antigen presenting cells. This property of HSPs suggests a potential function as an adjuvant-free carrier to stimulate immune responses against a covalently linked fusion partner. MAGE-A1 is a likely candidate for tumor immunotherapy due to its abundant immunogenic epitopes and strict tumor specificity. To analyze the influence of HSP70 conjugation to MAGE-A1, towards developing a novel effective vaccine against MAGE-expressing tumors, we cloned the murine counterpart of the human HSP70 and MAGE-A1 genes.

Methods

Recombinant proteins expressing Mage-a1 (aa 118-219), Hsp70, and Mage-a1-Hsp70 fusion were purified and used to immunize

SUBMITTER: Jiang J 

PROVIDER: S-EPMC4029478 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature