Dissecting the critical factors for thermodynamic stability of modular proteins using molecular modeling approach.

ABSTRACT: Repeat proteins have recently attracted much attention as alternative scaffolds to immunoglobulin antibodies due to their unique structural and biophysical features. In particular, repeat proteins show high stability against temperature and chaotic agents. Despite many studies, structural features for the stability of repeat proteins remain poorly understood. Here we present an interesting result from in silico analyses pursuing the factors which affect the stability of repeat proteins. Previously developed repebody structure based on variable lymphocytes receptors (VLRs) which consists of leucine-rich repeat (LRR) modules was used as initial structure for the present study. We constructed extra six repebody structures with varying numbers of repeat modules and those structures were used f

SUBMITTER: Lee Y 

PROVIDER: S-EPMC4029881 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature