Identification of microRNAs regulating the developmental pathways of bone marrow derived mast cells.

ABSTRACT:

Background

MicroRNAs (miRNAs) play important roles in leukocyte differentiation, although those utilised for specific programs and key functions remain incompletely characterised. As a global approach to gain insights into the potential regulatory role of miRNA in mast cell differentiation we characterised expression in BM cultures from the initiation of differentiation. In cultures enriched in differentiating mast cells we characterised miRNA expression and identified miRNA targeting the mRNA of putative factors involved in differentiation pathways and cellular identity. Detailed pathway analysis identified a unique miRNA network that is intimately linked to the mast cell differentiation program.

Methodology/principal findings

We identified 86 unique miRNAs with expression

SUBMITTER: Xiang Y 

PROVIDER: S-EPMC4029961 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature