Global maternal early pregnancy peripheral blood mRNA and miRNA expression profiles according to plasma 25-hydroxyvitamin D concentrations.

ABSTRACT:

Objective

We investigated associations of early pregnancy maternal vitamin D concentrations with differential gene expression and post-transcription regulation.

Method

Plasma 25-hydroxyvitamin D (25[OH]D) was measured among participants of a nested case-control study. Participants with low (<25.5 ng/ml) and high (≥31.7 ng/ml) 25[OH]D were identified among controls. Peripheral blood messenger RNA (mRNA) (N = 21) and microRNA (miRNA) (N = 13) expression studies were conducted among participants with low and high 25[OH]D concentrations. Differential expression between low/high groups were evaluated using Student's t-test, fold change, and SAM comparisons. We further investigated functions and functional relationships of differentially expressed mRNAs and targets of differential

SUBMITTER: Enquobahrie DA 

PROVIDER: S-EPMC4030419 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature