Species and genus level resolution analysis of gut microbiota in Clostridium difficile patients following fecal microbiota transplantation.

ABSTRACT:

Background

Clostridium difficile is an opportunistic human intestinal pathogen, and C. difficile infection (CDI) is one of the main causes of antibiotic-induced diarrhea and colitis. One successful approach to combat CDI, particularly recurrent form of CDI, is through transplantation of fecal microbiota from a healthy donor to the infected patient. In this study we investigated the distal gut microbial communities of three CDI patients before and after fecal microbiota transplantation, and we compared these communities to the composition of the donor's fecal microbiota. We utilized phylogenetic Microbiota Array, high-throughput Illumina sequencing, and fluorescent in situ hybridization to profile microbiota composition down to the genus and species level resolution.

Results

SUBMITTER: Shankar V 

PROVIDER: S-EPMC4030581 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature