HSP70 transgene directed motion to nuclear speckles facilitates heat shock activation.

ABSTRACT: Association and disassociation of gene loci with respect to specific nuclear compartments accompany changes in gene expression, yet little is known concerning the mechanisms by which this occurs or its functional consequences. Previously, we showed that tethering acidic activators to a peripheral chromosome site led to movement of the chromosome site away from the nuclear periphery, but the physiological relevance of this movement was unclear [1]. Nuclear speckles, or interchromatin granule clusters, are enriched in factors involved in RNA processing [2], and the association of a subset of active genes at their periphery suggests speckles may play a role in gene expression [3, 4]. Here, we show an actin-dependent association of HSP70 transgenes with nuclear speckles after heat shock. We vi

SUBMITTER: Khanna N 

PROVIDER: S-EPMC4030642 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature