Cellular extract facilitates nuclear reprogramming by altering DNA methylation and pluripotency gene expression.

ABSTRACT: The functional reprogramming of a differentiated cell to a pluripotent state presents potential beneficial applications in disease mechanisms and regenerative medicine. Epigenetic modifications enable differentiated cells to perpetuate molecular memory to retain their identity. Therefore, the aim of this study was to investigate the reprogramming modification of yak fibroblast cells that were permeabilized and incubated in the extracts of mesenchymal stem cells derived from mice adipose tissue [adipose-derived stem cells (ADSCs)]. According to the results, the treatment of ADSC extracts promoted colony formation. Moreover, pluripotent gene expression was associated with the loss of repressive histone modifications and increased global demethylation. The genes Col1a1 and Col1a2, which are t

SUBMITTER: Xiong XR 

PROVIDER: S-EPMC4030660 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature