Development and Application of Multidimensional HPLC Mapping Method for O-linked Oligosaccharides.

ABSTRACT: Glycosylation improves the solubility and stability of proteins, contributes to the structural integrity of protein functional sites, and mediates biomolecular recognition events involved in cell-cell communications and viral infections. The first step toward understanding the molecular mechanisms underlying these carbohydrate functionalities is a detailed characterization of glycan structures. Recently developed glycomic approaches have enabled comprehensive analyses of N-glycosylation profiles in a quantitative manner. However, there are only a few reports describing detailed O-glycosylation profiles primarily because of the lack of a widespread standard method to identify O-glycan structures. Here, we developed an HPLC mapping method for detailed identification of O-glycans including ne

SUBMITTER: Yagi H 

PROVIDER: S-EPMC4030830 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature