RNAi-microsponges form through self-assembly of the organic and inorganic products of transcription.

ABSTRACT: Inorganic nanostructures have been used extensively to package nucleic acids into forms useful for therapeutic applications. Here we report that the two products of transcription, RNA and inorganic pyrophosphate, can self-assemble to form composite microsponge structures composed of nanocrystalline magnesium pyrophosphate sheets (Mg₂P₂O₇•3.5H₂O) with RNA adsorbed to their surfaces. The microsponge particles contain high loadings of RNA (15-21 wt.%) that are protected from degradation and can be obtained through a rolling circle mechanism as large concatemers capable of mediating RNAi. The morphology of the RNAi microsponges is influenced by the time-course of the transcription reaction and interactions between RNA and the inorganic phase. Previous work demonstrated that polycations can be

SUBMITTER: Shopsowitz KE 

PROVIDER: S-EPMC4031615 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature