Mass spectrometry and next-generation sequencing reveal an abundant and rapidly evolving abalone sperm protein.

ABSTRACT: Abalone, a broadcast spawning marine mollusk, is an important model for molecular interactions and positive selection in fertilization, but the focus has previously been on only two sperm proteins, lysin and sp18. We used genomic and proteomic techniques to bring new insights to this model by characterizing the testis transcriptome and sperm proteome of the Red abalone Haliotis rufescens. One pair of homologous, testis-specific proteins contains a secretion signal and is small, abundant, and associated with the acrosome. Comparative analysis revealed that homologs are extremely divergent between species, and show strong evidence for positive selection. The acrosomal localization and rapid evolution of these proteins indicates that they play an important role in fertilization, and could be

SUBMITTER: Palmer MR 

PROVIDER: S-EPMC4031754 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature