Analysis of plant microbe interactions in the era of next generation sequencing technologies.

ABSTRACT: Next generation sequencing (NGS) technologies have impressively accelerated research in biological science during the last years by enabling the production of large volumes of sequence data to a drastically lower price per base, compared to traditional sequencing methods. The recent and ongoing developments in the field allow addressing research questions in plant-microbe biology that were not conceivable just a few years ago. The present review provides an overview of NGS technologies and their usefulness for the analysis of microorganisms that live in association with plants. Possible limitations of the different sequencing systems, in particular sources of errors and bias, are critically discussed and methods are disclosed that help to overcome these shortcomings. A focus will be on the

SUBMITTER: Knief C 

PROVIDER: S-EPMC4033234 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature