Gene expression pattern of cells from inflamed and normal areas of osteoarthritis synovial membrane.

ABSTRACT:

Objective

To compare the gene expression patterns of synovial cells from inflamed or normal/reactive areas of synovial membrane obtained from the same patient with osteoarthritis (OA).

Methods

At the time of total knee replacement, synovial tissues were obtained from 12 patients with knee OA. The inflammation status of the synovial membrane was characterized according to macroscopic criteria and classified as normal/reactive or inflamed. Biopsy samples were cultured separately for 7 days. Microarray gene expression profiling was performed on normal/reactive and inflamed areas. Western blot and immunohistochemistry were used to confirm the identified genes that were differentially expressed.

Results

We identified 896 genes that were differentially expressed between nor

SUBMITTER: Lambert C 

PROVIDER: S-EPMC4033569 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature