Wolbachia detection in insects through LAMP: loop mediated isothermal amplification.

ABSTRACT:

Background

The bacterium Wolbachia is a promising agent for the biological control of vector-borne diseases as some strains have the ability to block the transmission of key human disease-causing pathogens. Fast, accurate and inexpensive methods of differentiating between infected and uninfected insects will be of critical importance as field-based trials of Wolbachia-based bio-control become increasingly common.

Findings

We have developed a specific and sensitive method of detecting Wolbachia based on the isothermal DNA amplification. This technique can be performed in an ordinary heat block without the need for gel-based visualisation, and is effective for a wide variety of insect hosts.

Conclusion

Here we present the development of a rapid, highly sensitive and ine

SUBMITTER: Goncalves Dda S 

PROVIDER: S-EPMC4033683 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature