Association between heat shock protein 70 gene polymorphisms and clinical outcomes in intensive care unit patients with sepsis.

ABSTRACT:

Objective

The objective of the following study is to evaluate the associations between single nucleotide polymorphisms (SNPs) in the Heat Shock Protein 70 (HSP70) gene, gene expression of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) and medical intensive care unit (MICU) stay and organ failure in sepsis.

Materials and methods

MICU patients with sepsis were genotyped for rs1061581, rs2227956, rs1008438 and rs1043618 polymorphisms in HSP70 gene using polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis or allele-specific PCR. Messenger ribonucleic acid (mRNA) expression of IL-6 and TNF-α were quantitated in peripheral blood lymphocytes. Outcomes were recorded.

Results

108 patients (48 male) aged 40.7 ± 16.0 (mean ± standard d

SUBMITTER: Ramakrishna K 

PROVIDER: S-EPMC4033853 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature