Postnatal lineage mapping of follicular melanocytes with the Tyr::CreER(T) (2) transgene.

ABSTRACT: One of the main advantages of using inducible and conditional transgenes to study pigment cell biology is that they allow for genetic manipulation within melanocytes after roles in general neural crest or melanoblast development have been fulfilled. Specifically, we focus here on the ability of the Tyr::CreER(T) (2) transgenic line to alter genes within follicular melanocytes postnatally. Using the Gt(ROSA)26Sor(tm1sor) reporter allele, we present in detail the expression and activity of Tyr::CreER(T) (2) when induced during hair morphogenesis and adult hair cycling. We find that despite similarities in expression pattern to endogenous TYR, Tyr::CreER(T) (2) is effective at targeting both undifferentiated and differentiated melanocytes within the hai

SUBMITTER: Harris ML 

PROVIDER: S-EPMC4034131 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature