Antisense-mediated FLC transcriptional repression requires the P-TEFb transcription elongation factor.

ABSTRACT: The functional significance of noncoding transcripts is currently a major question in biology. We have been studying the function of a set of antisense transcripts called COOLAIR that encompass the whole transcription unit of the Arabidopsis floral repressor FLOWERING LOCUS C (FLC). Alternative polyadenylation of COOLAIR transcripts correlates with different FLC sense expression states. Suppressor mutagenesis aimed at understanding the importance of this sense-antisense transcriptional circuitry has identified a role for Arabidopsis cyclin-dependent kinase C (CDKC;2) in FLC repression. CDKC;2 functions in an Arabidopsis positive transcription elongation factor b (P-TEFb) complex and influences global RNA polymerase II (Pol II) Ser(2) phosphorylation levels. CDKC;2 activity directly promote

SUBMITTER: Wang ZW 

PROVIDER: S-EPMC4034230 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature