Technological advances in bifidobacterial molecular genetics: application to functional genomics and medical treatments.

ABSTRACT: Bifidobacteria are well known as beneficial intestinal bacteria that exert health-promoting effects in humans. In addition to physiological and immunological investigations, molecular genetic technologies have been developed and have recently started to be applied to clarify the molecular bases of host-Bifidobacterium interactions. These technologies include transformation technologies and Escherichia coli-Bifidobacterium shuttle vectors that enable heterologous gene expression. In this context, a plasmid artificial modification method that protects the introduced plasmid from the restriction system in host bifidobacteria has recently been developed to increase transformation efficiency. On the other hand, targeted gene inactivation systems, which are vital for functional genomics, seemed

SUBMITTER: Fukiya S 

PROVIDER: S-EPMC4034290 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature