Deep-sequencing transcriptome analysis of low temperature perception in a desert tree, Populus euphratica.

ABSTRACT:

Background

Compared with other Populus species, Populus euphratica Oliv. exhibits better tolerance to abiotic stress, especially those involving extreme temperatures. However, little is known about gene regulation and signaling pathways involved in low temperature stress responses in this species. Recent development of Illumina/Solexa-based deep-sequencing technologies has accelerated the study of global transcription profiling under specific conditions. To understand the gene network controlling low temperature perception in P. euphratica, we performed transcriptome sequencing using Solexa sequence analysis to generate a leaf transcriptome at a depth of 10 gigabases for each sample.

Results

Using the Trinity method, 52,081,238 high-quality trimmed reads were assembled into

SUBMITTER: Chen J 

PROVIDER: S-EPMC4035058 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature