Discovery of transgene insertion sites by high throughput sequencing of mate pair libraries.

ABSTRACT:

Background

Transgenesis by random integration of a transgene into the genome of a zygote has become a reliable and powerful method for the creation of new mouse strains that express exogenous genes, including human disease genes, tissue specific reporter genes or genes that allow for tissue specific recombination. Nearly 6,500 transgenic alleles have been created by random integration in embryos over the last 30 years, but for the vast majority of these strains, the transgene insertion sites remain uncharacterized.

Results

To obtain a complete understanding of how insertion sites might contribute to phenotypic outcomes, to more cost effectively manage transgenic strains, and to fully understand mechanisms of instability in transgene expression, we've developed methodology an

SUBMITTER: Srivastava A 

PROVIDER: S-EPMC4035081 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature